Mycobactin J

Antigen (PPA) 

Serology

Milk ELISA

Research

The Paratuberculosis Laboratory
"Where Research Is Applied"

Allied Monitor Testing Services

Allied's SELISA and Fecal Culture Testing Services are USDA, 
NVSL Check Test Approved and Certified.

 

Serum ELISA (SELISA)

The Serum ELISA (enzyme-linked immunosorbent assay) detects circulating antibodies to Mycobacterium avium ssp. paratuberculosis (MAP) in serum from a blood sample. Allied Monitor's SELISA has a reported specificity of 95.4% and a sensitivity of 58.8%.  A confirmative AGID test is performed for all positive SELISA results, unless no AGID test is requested.

Depressed serological titers due to late pregnancy and anergy due to advanced paratuberculosis are not uncommon resulting in the possibility of a false negative SELISA result.  Under these conditions the use of the AGID test will most always detect the presence of antibodies to MAP resulting in a true positive test.  Why the antibody is detected in AGID and not SELISA under these conditions is unknown to researchers at this time.  If an animal is in pregnancy and/or showing clinical signs of Johne's Disease, an AGID test is highly recommended for that individual.  Please be sure to note the test animal's health condition when submitting sample.

In most instances, Allied's SELISA result can be reported within 24 hours of sample receipt.  For more than 300 samples, please notify us prior to shipping.

The Milk ELISA  (enzyme-linked immunosorbent assay) detects antibodies to Mycobacterium avium ssp. paratuberculosis (MAP) in milk samples.  The Milk ELISA is a fast, convenient, and economic method for the annual or semiannual screening of dairy herds (bulk tank) or individual animals. Allied's Kinetic Bilk Tank MELISA may include up to 50 individuals per sample.  Please be sure to note number of individuals per bulk tank sample on submission form.  If elevated titers are detected during consecutive bank tank sampling, then "Escalation Testing" would be recommended by testing each new addition with individual SELISA and/or Fecal Culture.  The intended purpose of the individual MELISA and Kinetic Bulk Tank MELISA is to be used as an inexpensive screening tool to monitor the status of a dairy herd and should not be confused with or used as a true diagnostic test.

An in-house evaluation indicated a 92.8% agreement for MELISA to the SELISA specificity and sensitivity.

In most instances, Allied's MELISA results can be reported within 24 hours of sample receipt.  For more than 300 samples, please notify us prior to shipping.

The AGID (Agar Gel Immunodiffusion) is used to support a positive SELISA result or may be used as a separate test. AGID specificity is >99%.  Under conditions of late pregnancy and/or clinical signs of Johne's Disease, the use of an AGID test is highly recommended.

AGID results are reported after 48 hours of sample receipt.  For more than 300 samples, please notify us prior to shipping.

Fecal culture remains the gold standard in Johne's Testing.  A positive fecal culture confirms Mycobacterium avium ssp. paratuberculosis (MAP).  Since the organisms are shed intermittently, a negative result does not rule out the presence of disease.  Fecal culturing has a reported specificity of 100% and a sensitivity of 40% to 50%.  Routine testing is highly recommended.

Culture results are reported after 8 weeks of incubation.    For more than 300 samples, please notify us prior to shipping.

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